LOCUS p201G Cas9 13943 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to
accept gRNA from pUC gRNA Shuttle.
ACCESSION .
VERSION .
KEYWORDS p201G Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13943)
AUTHORS Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA.
TITLE Targeted genome modifications in soybean with CRISPR/Cas9.
JOURNAL BMC Biotechnology. 2015;15:16
REFERENCE 2 (bases 1 to 13943)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13943
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1187..1816
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2250..3317
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 3383..3577
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(3813..3835)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3921..4061
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3976..3995
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4076..4093)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4247..4835)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4327..4346)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4930..5745)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 5653..5672
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 5737..5756
/label=pENTR-R
/note="pENTR vectors, reverse primer"
misc_feature 6038..6062
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
protein_bind 6239..6260
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6275..6305
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6313..6329
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6318..6340
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6337..6353
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6337..6353
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
terminator 6409..6661
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(6680..7399)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(6725..6746)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 7074..7093
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7333..7354
/label=EGFP-N
/note="EGFP, reverse primer"
promoter complement(7445..8121)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 8375..9051
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
CDS 9097..13200
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 13213..13233
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 13300..13552
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(13594..13611)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(13594..13610)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(13603..13625)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 13806..13830
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg caggatcatg
4921 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
4981 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
5041 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
5101 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
5161 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
5221 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
5281 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
5341 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
5401 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
5461 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
5521 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
5581 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
5641 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
5701 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
5761 actgtttatg taagcagaca gttttattgt tcatgatctg gatcacaggc agcaacgctc
5821 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
5881 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
5941 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
6001 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
6061 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
6121 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
6181 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
6241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
6301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
6361 cgaattcctt gactagaggg taggcgcggc ttaattaaat caattcccga tctagtaaca
6421 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
6481 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
6541 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
6601 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
6661 ctgcagccgg gcggccgctt tacttgtaca gctcgtccat gccgtgagtg atcccggcgg
6721 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
6781 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
6841 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
6901 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
6961 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
7021 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
7081 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
7141 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
7201 aggtgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
7261 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
7321 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
7381 cctcgccctt gctcaccatg gatccggtcg agagagatag atttgtagag agagactggt
7441 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
7501 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
7561 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
7621 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
7681 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
7741 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
7801 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
7861 ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
7921 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
7981 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
8041 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
8101 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
8161 ttggagtaga cgagagtgtc gtgctccacc atgttgggac tagtgagtcg tattacaatt
8221 catgactctc ttaaggtagc caaaatcgat atctagggat aacagggtaa tcgatcacta
8281 gaattcggcg cgccgggccc aacatggtgg agcacgacac tctcgtctac tccaagaata
8341 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
8401 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
8461 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
8521 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
8581 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac
8641 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
8701 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
8761 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
8821 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
8881 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
8941 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
9001 gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag
9061 tctctctcta caaatctatc tctctcgacc gctagcatgg acaagaagta ctccattggg
9121 ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg
9181 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt
9241 ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa aagaacagca
9301 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat
9361 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag
9421 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac
9481 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag
9541 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc
9601 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg
9661 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc
9721 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag
9781 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg
9841 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa
9901 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac
9961 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg
10021 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac
10081 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag
10141 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc
10201 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag
10261 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga
10321 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat
10381 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata
10441 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa
10501 tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc
10561 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt
10621 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa
10681 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc
10741 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagactat
10801 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac
10861 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac
10921 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat
10981 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg
11041 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat
11101 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt
11161 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc
11221 cagaaagcac aagtttctgg ccagggggac agtcttcacg agcacatcgc taatcttgca
11281 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc
11341 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa
11401 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata
11461 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat
11521 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg
11581 gacatcaatc ggctctccga ctacgacgtg gatcatatcg tgccccagtc ttttctcaaa
11641 gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat
11701 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac
11761 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg
11821 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc
11881 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa
11941 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtctcaga tttcagaaag
12001 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac
12061 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt
12121 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa
12181 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc
12241 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa
12301 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc
12361 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa
12421 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc
12481 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa
12541 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc
12601 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa
12661 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac
12721 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg
12781 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaagggtct
12841 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag
12901 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat
12961 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac
13021 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac
13081 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt
13141 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac
13201 agcagggctg accccaagaa gaagaggaag gtgtgaccgc ggatatctgc agaagcttac
13261 gcgtcgacgt ctcgaggccg gccgagctcg aatttccccg atcgttcaaa catttggcaa
13321 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
13381 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
13441 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
13501 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
13561 atatcgtagg gataacaggg taataagctt ggcactggcc gtcgttttac aacgtcgtga
13621 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
13681 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
13741 tggcgaatga gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
13801 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac
13861 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
13921 cacagggttc ccctcgggat caa
//
